Helping researchers face the data analysis challenge

Our knowledge of each technology’s potential and limitations from a bioinformatic perspective ultimately saves users time and precious funds. Request services

Services

C3G offers a wide range of standardized and tailored analysis services to researchers, who in turn benefit from our extensive experience analyzing data from various sequencing applications with state of the art computation methods.

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At C3G we provide customized and case-by-case analysis services!
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DNA-seq

C3G offers state of the art DNA-seq analyses detects and annotates variants in whole exomes, whole genomes or high coverage amplicons. The analysis can also be pushed further by assisting with variant prioritization, or perform advanced cancer related analysis.

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RNA-seq de novo

This analysis assembles reads to transcripts in the absence of a reference genome, annotates transcripts and tests for differential expression. This is well suited to uncharacterized organisms.

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rRNA amplicon-seq metagenomics

The C3G can process your Illumina, PacBio pyrotags amplicons from the 16S, 18S or ITS amplicons. OTUs are picked and diversity is analyzed within and between communities. Further analyses include differential abundance testing or metagenome functional content prediction.

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Hybrid assembly of large genomes

The C3G can perform de novo assemblies of genomes using Illumina paired-end reads, possibly supplemented with long insert mate-pairs and lower coverage PacBio long reads to scaffold contigs and fill gaps.

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miRNA-seq

C3G’s standard miRNA analysis quantifies know miRNAs, discovers novel ones using a reference genome and performs differential expression analysis. Additional analysis may include pathway testing, target candidates analysis or miRNA editing.

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RNA-seq

In this application, C3G will quantify transcripts and genes using a reference genome and test for differential expression across experimental conditions. It is also possible to call single nucleotide variants (SNVs), detect fusion gene events and assess alternative splicing events.

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PacBio genome assembly

C3G offers to assemble small bacterial to larger genomes sequenced with the Pacific Biosciences technology. Genomes can be polished with Illumina short reads, circularized, called for base modifications and annotated.

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ChIP-seq

Our team analyzes DNA fragments from immunoprecipitated chromatin by calling alignment peaks on the genome, annotating the said peaks and performing additional analyses such as motif enrichment and discovery. Designed experiments can be analyzed by testing for differential binding between experimental conditions.

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DNA methylation

Our team offers to analyze data coming from bisulfite converted DNA assayed by various sequencing assays such as RRBS, CpG capture, whole genome sequencing or microarrays. Our analysis computes methylation levels and performs differential analysis between experimental conditions.

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Expression microarrays

C3G’s standard analysis will QC, preprocess and perform differential expression analysis on expression array data. This is usually followed by pathway analysis and the preparation of custom visualizations.

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Other Analyses

  • CRISPR-Cas9 processing and statistical analysis
  • Pool-seq
  • GBS, RAD-Seq
  • Whole metagenome/metatranscriptome analysis

Pricing

Charges for services are calculated based on an hourly rate of $68.00 (CAD) for canadian academic and nonprofit organizations, $77.00 (CAD) for non-canadian academic and nonprofit organizations, and $95.00 (CAD) for for-profit corporations.

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Want to know more about our services and how we can help! Send us an inquiry and we will be happy to answer your questions.

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